DNA Methylation: Bisulphite Modification and Analysis
نویسندگان
چکیده
منابع مشابه
Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA.
Methylation analysis of individual cytosines in genomic DNA can be determined quantitatively by bisulphite treatment and PCR amplification of the target DNA sequence, followed by restriction enzyme digestion or sequencing. Methylated and unmethylated molecules, however, have different sequences after bisulphite conversion. For some sequences this can result in bias during the PCR amplification ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2011
ISSN: 1940-087X
DOI: 10.3791/3170